Build your sequencer

Build your sequencer

Meet the Transformer: 454 Bio’s One Pot sequencing instrument. At its core, the Transformer is an automated temperature controlled LED-TIRF microscope . Its main components are:

  • Temperature-controlled main body
  • High-power LEDs for excitation and photocleavage of Lightning Terminators™
  • Custom optics: automated focus adjustment and filter selection
  • Off-the-shelf camera sensor
  • Raspberry Pi computer with custom software and electronics to control and collect data

This section describes everything you will need to build your own sequencer.

Hardware: Transformer Mark 3

Hardware Guide


Electronics Guide

Instrument software

Microscope control software for automated data collection