Build your sequencer
Meet the Transformer: 454 Bio’s One Pot sequencing instrument. At its core, the Transformer is an automated temperature controlled LED-TIRF microscope . Its main components are:
- Temperature-controlled main body
- High-power LEDs for excitation and photocleavage of Lightning Terminators™
- Custom optics: automated focus adjustment and filter selection
- Off-the-shelf camera sensor
- Raspberry Pi computer with custom software and electronics to control and collect data
This section describes everything you will need to build your own sequencer.