Build your sequencer

Meet the Transformer: 454 Bio’s One Pot sequencing instrument. At its core, the Transformer is an automated temperature controlled LED-TIRF microscope . Its main components are:

• Temperature-controlled main body
• High-power LEDs for excitation and photocleavage of Lightning Terminators™
• Custom optics: automated focus adjustment and filter selection
• Off-the-shelf camera sensor
• Raspberry Pi computer with custom software and electronics to control and collect data

This section describes everything you will need to build your own sequencer.